Journal: Heliyon

Article Title: Human intestine and placenta exhibit tissue-specific expression of RAGE isoforms.

doi: 10.1016/j.heliyon.2023.e18247

Figure Lengend Snippet: Fig. 5. RAGE immunoblotting analysis of human intestine. SDS-PAGE of lung lysate (1.5 μg/lane) as well as lysates from small intestine (SI) and colon (50 μg/lane) was performed under reducing conditions. Membranes were stained with three different anti-RAGE antibodies; SC A11 (A), AF1145 (B), and AB D01P (C). Membranes were also stained with the respective secondary antibodies only (negative control). GAPDH was used as a loading control. The uncropped images are shown in the supplementary material of the manuscript.

Article Snippet: Human Lung Whole Tissue Lysate (Adult Whole Normal) was obtained from Novus Biologicals and protein extracts from healthy human small intestine and colon were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot, SDS Page, Staining, Negative Control, Control

Journal: Heliyon

Article Title: Human intestine and placenta exhibit tissue-specific expression of RAGE isoforms.

doi: 10.1016/j.heliyon.2023.e18247

Figure Lengend Snippet: Fig. 4. (A) RAGE immunoblotting analysis of human lung lysate. SDS-PAGE of lung lysate (1 μg/lane; PNGase F -) and deglycosylated lung lysate (1 μg/lane; PNGase F +) was performed under reducing conditions. Membranes were stained with four different anti-RAGE antibodies. Membranes were also stained with the respective secondary antibodies only (negative control). GAPDH was used as a loading control. (B) RAGE immunoblotting analysis of RAGE overexpressing (+) and control (−) HEK293T cells. SDS-PAGE was performed under reducing conditions. For WB analysis membranes were stained with anti-FLAG and anti-RAGE (AB D01P) antibodies. The uncropped images are shown in the supplementary material of the manuscript.

Article Snippet: Human Lung Whole Tissue Lysate (Adult Whole Normal) was obtained from Novus Biologicals and protein extracts from healthy human small intestine and colon were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot, SDS Page, Staining, Negative Control, Control

Journal: Heliyon

Article Title: Human intestine and placenta exhibit tissue-specific expression of RAGE isoforms.

doi: 10.1016/j.heliyon.2023.e18247

Figure Lengend Snippet: Fig. 6. RAGE immunoblotting analysis of human placenta derived from different healthy (H) as well as GDM-, FGR- and PE-affected pregnancies. SDS-PAGE of lung lysate (1 μg/lane) as well as lysates from the placentas (25 μg/lane) was performed under reducing conditions. For WB analysis membranes were stained with three different anti-RAGE antibodies; SC A11 (A), AF1145 (B), and AB D01P (C). Membranes were also stained with the respective secondary antibodies only (negative control). Ponceau S staining was used as a loading control. The uncropped images are shown in the supplementary material of the manuscript.

Article Snippet: Human Lung Whole Tissue Lysate (Adult Whole Normal) was obtained from Novus Biologicals and protein extracts from healthy human small intestine and colon were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot, Derivative Assay, SDS Page, Staining, Negative Control, Control

Journal: eLife

Article Title: Sperm-specific COX6B2 enhances oxidative phosphorylation, proliferation, and survival in human lung adenocarcinoma

doi: 10.7554/eLife.58108

Figure Lengend Snippet: ( A–C ) Kaplan-Meier curves for OS and FP in NSCLC ( A ) LUAD ( B ) and LUSC patients ( C ). ( D ) COX6B2 mRNA expression (RNA-seq RSEM, log2(norm count +1)) from TCGA Lung Cancer dataset. Bars represent median (normal: n = 110; tumor: n = 1017). P values calculated by Mann-Whitney test. ( E–F ) Whole tissue homogenates of LUAD tumors ( E ) and LUAD cell line lysates ( F ) were immunoblotted with indicated antibodies. Molecular weight (MW) markers are indicated. ( G ) IHC staining of non-malignant testis (a positive control), non-malignant lung (adjacent normal) and LUAD tissues. Scores ranged from 0 to 3. Scale bar, 50 μm. Bars represent mean ± SEM. p-Values calculated by Mann-Whitney test. ( H ) Representative confocal images of endogenous COX6B2 in HCC515 cells. Tom20 is used as a mitochondrial marker. Images were shown as Z-stack maximum projection from 0.3-μm-thick image. Scale bar, 10 μm.

Article Snippet: Human normal lung whole tissue lysate was purchased from Novus Biologicals (NB820-59237).

Techniques: Expressing, RNA Sequencing, MANN-WHITNEY, Molecular Weight, Immunohistochemistry, Positive Control, Marker

Journal:

Article Title: Phosphorylated Eukaryotic Translation Initiation Factor 4 (eIF4E) is Elevated in Human Cancer Tissues

doi:

Figure Lengend Snippet: Comparison of p-eIF4E expression between the tumors and adjacent normal tissues

Article Snippet: Human lung cancer TMA consisting of 40 cases of stage I-III lung cancer tissues, 10 cases of metastatic cancer tissues from the primary lung cancer, and 9 cases of adjacent normal human lung tissues was purchased from Imgenex (IMH-358; SanDiego, CA).

Techniques: Comparison, Expressing, Wilms Tumor Assay

Journal: BMC Cancer

Article Title: CIP2A overexpression induces autoimmune response and enhances JNK signaling pathway in human lung cancer

doi: 10.1186/s12885-015-1899-0

Figure Lengend Snippet: Frequency of autoantibody in sera from patients with lung cancer

Article Snippet: Formalin fixed and paraffin embedded lung cancer tissue arrays and normal human lung tissue were obtained from Cybrdi (Rockville, MD).

Techniques:

Journal: BMC Cancer

Article Title: CIP2A overexpression induces autoimmune response and enhances JNK signaling pathway in human lung cancer

doi: 10.1186/s12885-015-1899-0

Figure Lengend Snippet: CIP2A elicits autoimmune response in lung cancer patients. a Representative Western-blot results of autoantigen screening using human sera. A549 total cell lysates were resolved on 12 % SDS-PAGE gel, and transferred to nitrocellular membrane. The membrane was then cut into stripes to detect the presence of autoantibody in individual human sera. Figure shows lung cancer sera contained autoantibody against a band between 75 kD and 100 kD (serum 6 and serum 52). NHS: normal human sera; LC: lung cancer. b Validation of CIP2A autoantigen. Five randomly chosen CIP2A positive sera were used for this validation. Antibodies against CIP2A containing sera were firstly depleted with recombinant GST-CIP2A protein, which was then used for Western-blot for CIP2A fusion protein resolved on SDS-PAGE gel

Article Snippet: Formalin fixed and paraffin embedded lung cancer tissue arrays and normal human lung tissue were obtained from Cybrdi (Rockville, MD).

Techniques: Western Blot, SDS Page, Membrane, Biomarker Discovery, Recombinant

Journal: BMC Cancer

Article Title: CIP2A overexpression induces autoimmune response and enhances JNK signaling pathway in human lung cancer

doi: 10.1186/s12885-015-1899-0

Figure Lengend Snippet: Frequency of autoantibody to CIP2A in sera from patients with lung cancer

Article Snippet: Formalin fixed and paraffin embedded lung cancer tissue arrays and normal human lung tissue were obtained from Cybrdi (Rockville, MD).

Techniques:

Journal: BMC Cancer

Article Title: CIP2A overexpression induces autoimmune response and enhances JNK signaling pathway in human lung cancer

doi: 10.1186/s12885-015-1899-0

Figure Lengend Snippet: CIP2A expression in normal lung tissue and lung cancer tissue by immunohistochemistry (IHC). Representative IHC images of human lung cancer tissues and normal human lung tissues using monoclonal mouse anti-CIP2A antibody. Column 1 includes H&E stained lung tissues to show the histology of the specimen. Colum 2 showed the IHC results from normal lung tissue, Grade II lung cancer tissue and Grade III lung tissues. Column 3 is the enlarged view of column 2, which is shown as squared region. All scale bars are 50 μm

Article Snippet: Formalin fixed and paraffin embedded lung cancer tissue arrays and normal human lung tissue were obtained from Cybrdi (Rockville, MD).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: BMC Cancer

Article Title: CIP2A overexpression induces autoimmune response and enhances JNK signaling pathway in human lung cancer

doi: 10.1186/s12885-015-1899-0

Figure Lengend Snippet: IHC results showing expression level of CIP2A in lung cancer tissue specimens

Article Snippet: Formalin fixed and paraffin embedded lung cancer tissue arrays and normal human lung tissue were obtained from Cybrdi (Rockville, MD).

Techniques: Expressing

Journal: Cancer Science

Article Title: Radioimmunotherapy of human synovial sarcoma using a monoclonal antibody against FZD10

doi: 10.1111/j.1349-7006.2007.00701.x

Figure Lengend Snippet: Immunohistochemical staining analyses of synovial sarcoma and normal human tissue sections using MAb 92‐13; (a,b) synovial sarcoma tissue sections (c) colon, (d) heart, (e) lung, (f) liver, (g) kidney and (h) placenta. Original magnification ×100. Bar, 50 µm.

Article Snippet: For immunohistochemical staining analysis, 4‐µm sections of paraffin‐embedded normal adult human tissues (lung, heart, liver, kidney, colon and placenta) (BioChain, Hayward, CA, USA) and surgical synovial sarcoma specimens were deparaffinized and processed for antigen retrieval in Target Retrieval Solution (pH 9) (DAKO Cytomation, Carpinteria, CA, USA) at 125°C for 30 s. After treatment with peroxidase blocking reagent (DAKO Cytomation) for 10 min, followed by treatment with protein blocking serum‐free (DAKO Cytomation) for 30 min, slides were incubated with 10 µg/mL MAb 92‐13.

Techniques: Immunohistochemical staining, Staining